Pectinase Production by Aspergillus niger using Pineapple Peel Pectin and Its Application in Coconut Oil Extraction

Abstract Pectinases, like other industrial enzymes are usually expensive. The use of pineapple peel pectin as substrate is triggered by the large tones waste generated in Nigeria. Oil extraction mechanical/chemical means have associated disadvantages. This research aimed at employing locally produced pectinase for coconut-oil and to compare yield with commercial pectinase. Fifty grammes dried powder were employed production. Aspergillus niger isolated from cassava meal was produce using submerged fermentation seven days. activity determined 24 h interval. partially purified 3% activated carbon, characterized extract oil coconut. peels 24.8% after 1 time. Highest observed on day five. Optimum conditions 40°C, 5.0 1% respectively temperature, pH concentration. enzyme completely inactive 5 min heating 90°C metal ion (Mg 2+) stimulated its activity. mean greater than this study enhanced when compared mechanical method.